Extensive local adaptation within the chemosensory system following Drosophila melanogaster's global expansion.

How organisms adapt to new environments is of fundamental biological interest, but poorly understood at the genetic level. Chemosensory systems provide attractive models to address this problem, because they lie between external environmental signals and internal physiological responses. To investigate how selection has shaped the well-characterized chemosensory system of Drosophila melanogaster, we have analysed genome-wide data from five diverse populations. By couching population genomic analyses of chemosensory protein families within parallel analyses of other large families, we demonstrate that chemosensory proteins are not outliers for adaptive divergence between species. However, chemosensory families often display the strongest genome-wide signals of recent selection within D. melanogaster. We show that recent adaptation has operated almost exclusively on standing variation, and that patterns of adaptive mutations predict diverse effects on protein function. Finally, we provide evidence that chemosensory proteins have experienced relaxed constraint, and argue that this has been important for their rapid adaptation over short timescales

Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin productioninfo:eu-repo/semantics/publishedVersio

Generalized contact process on random environments

Spreading from a seed is studied by Monte Carlo simulation on a square lattice with two types of sites affecting the rates of birth and death. These systems exhibit a critical transition between survival and extinction. For time- dependent background, this transition is equivalent to those found in homogeneous systems (i.e. to directed percolation). For frozen backgrounds, the appearance of Griffiths phase prevents the accurate analysis of this transition. For long times in the subcritical region, spreading remains localized in compact (rather than ramified) patches, and the average number of occupied sites increases logarithmically in the surviving trials.Comment: 6 pages, 7 figure

Field theoretic description of charge regulation interaction

In order to find the exact form of the electrostatic interaction between two proteins with dissociable charge groups in aqueous solution, we have studied a model system composed of two macroscopic surfaces with charge dissociation sites immersed in a counterion-only ionic solution. Field-theoretic representation of the grand canonical partition function is derived and evaluated within the mean-field approximation, giving the Poisson-Boltzmann theory with the Ninham-Parsegian boundary condition. Gaussian fluctuations around the mean-field are then analyzed in the lowest order correction that we calculate analytically and exactly, using the path integral representation for the partition function of a harmonic oscillator with time-dependent frequency. The first order (one loop) free energy correction gives the interaction free energy that reduces to the zero-frequency van der Waals form in the appropriate limit but in general gives rise to a mono-polar fluctuation term due to charge fluctuation at the dissociation sites. Our formulation opens up the possibility to investigate the Kirkwood-Shumaker interaction in more general contexts where their original derivation fails.Comment: 12 pages, 9 figures, submitted to EPJ

Debate sobre o artigo de Narvai et al.

English title: Debate on the paper by Narvai et al.Margareth Crisóstomo Portela & Mauricio Teixeira Leite de Vasconcello

Colocalization of the (Pro)renin receptor/Atp6ap2 with H+-AT pases in mouse kidney but prorenin does not acutely regulate intercalated cell H+-ATPase activity

The (Pro)renin receptor (P)RR/Atp6ap2 is a cell surface protein capable of binding and nonproteolytically activate prorenin. Additionally, (P)RR is associated with H+-ATPases and alternative functions in H+-ATPase regulation as well as inWnt signalling have been reported. Kidneys express very high levels of H+-ATPases which are involved in multiple functions such as endocytosis, membrane protein recycling as well as urinary acidification, bicarbonate reabsorption, and salt absorption. Here, we wanted to localize the (P)RR/Atp6ap2 along the murine nephron, exmaine whether the (P)RR/Atp6ap2 is co

Motion of influential players can support cooperation in Prisoner's Dilemma

We study a spatial Prisoner's dilemma game with two types (A and B) of players located on a square lattice. Players following either cooperator or defector strategies play Prisoner's Dilemma games with their 24 nearest neighbors. The players are allowed to adopt one of their neighbor's strategy with a probability dependent on the payoff difference and type of the given neighbor. Players A and B have different efficiency in the transfer of their own strategy therefore the strategy adoption probability is reduced by a multiplicative factor (w < 1) from the players of type B. We report that the motion of the influential payers (type A) can improve remarkably the maintenance of cooperation even for their low densities.Comment: 7 pages, 7 figure

Novel universality class of absorbing transitions with continuously varying critical exponents

The well-established universality classes of absorbing critical phenomena are directed percolation (DP) and directed Ising (DI) classes. Recently, the pair contact process with diffusion (PCPD) has been investigated extensively and claimed to exhibit a new type of critical phenomena distinct from both DP and DI classes. Noticing that the PCPD possesses a long-term memory effect, we introduce a generalized version of the PCPD (GPCPD) with a parameter controlling the memory effect. The GPCPD connects the DP fixed point to the PCPD point continuously. Monte Carlo simulations show that the GPCPD displays novel type critical phenomena which are characterized by continuously varying critical exponents. The same critical behaviors are also observed in models where two species of particles are coupled cyclically. We suggest that the long-term memory may serve as a marginal perturbation to the ordinary DP fixed point.Comment: 13 pages + 10 figures (Full paper version